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KMID : 0613820130230050609
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Journal of Life Science
2013 Volume.23 No. 5 p.609 ~ p.615
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An Investigation of Glyceollin I's Inhibitory Effect on The Mammalian Adenylyl
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Kim Dong-Chan
Kim Nam-Doo
Kim Sung-In
Jang Chul-Soo
Kweon Chang-Oh
Kim Byung-Weon
Ryu Jae-Ki
Kim Hyun-Kyung
Lee Suk-Jun
Lee Seung-ho
Kim Dong-jin
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Abstract
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Glyceollin I has gained attention as a useful therapy for various dermatological diseases. However, the binding property of glyceollin I to the mammalian adenylyl cyclase (hereafter mAC), a critical target enzyme for the down-regulation of skin melanogenesis, has not been fully explored. To clarify the action mechanism between glyceollin I and mAC, we first investigated the molecular docking property of glyceollin I to mAC and compared with that of SQ22,536, a well-known mAC inhibitor, to mAC. Glyceollin I showed superiority by forming three hydrogen bonds with Asp 1018, Trp 1020, and Asn 1025, which exist in the catalytic site of mAC. However, SQ22,536 formed only two hydrogen bonds with Asp 1018 and Asn 1025. Secondly, we confirmed that glyceollin I effectively inhibits the formation of forskolin-induced cAMP and the phosphorylation of PKA from a cell-based assay. Long term treatment with glyceollin I had little effect on the cell viability. The findings of the present study also suggest that glyceollin I may be extended to be used as an effective inhibitor of hyperpigmentation.
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KEYWORD
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Glyceollin I, mammalian adenylyl cyclase, SQ22536, melanogenesis, computation modeling
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